HPLC · CoA
How Do You Reconstitute a BPC-157 + TB-500 Blend?
Guide · 9 min read · Updated 30 September 2026
To reconstitute a BPC-157 + TB-500 blend, bring the sealed vial to room temperature, swab the stopper, add bacteriostatic water gradually along the inner glass and swirl until the cake has fully dissolved. Two millilitres in the 5+5mg vial yields a combined 5 mg per mL, or 2.5 mg per mL for each peptide. The 10+10mg vial reaches the same per-peptide strength at 4 mL. Refrigerate the resulting stock between 2 and 8 °C, labelled with the date and both concentrations.
We supply the pair as a co-lyophilised BPC-157 and TB-500 blend in 5+5mg and 10+10mg vials, each lot verified by HPLC at 98% or higher, with mass spectrometry confirming identity. Every calculation for a blend rests on two figures held together: the combined strength of the solution and the share of that strength belonging to each compound. Keep both in the lab record and every chart and syringe reading follows directly.
What Is BPC-157/TB-500 in a Blend Vial?
A BPC-157/TB-500 blend is two unrelated research peptides freeze-dried together in equal weights under a single lot number. BPC-157 is a synthetic pentadecapeptide, 15 residues long (Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val), with a molecular weight near 1,419 daltons. Its sequence derives from a larger gastric protein, and it is unusually stable in acid for a linear peptide. TB-500 is the trade name for a synthetic sequence based on thymosin beta-4, the 43 residue actin-binding protein of about 4,963 daltons whose sequence appeared in PNAS in 1981. Some material sold under that name is the full chain and some is a short fragment built around the LKKTETQ actin-binding motif, so the lot's certificate of analysis is the document that confirms which form is present.
No chemical bond joins the two inside the vial. They share a cake, a label and a lot number, and they dissolve into one solution. Laboratories choose the combination because the compounds are studied on separate strands of tissue repair biology: BPC-157 in angiogenesis signalling models involving VEGFR2 and nitric oxide, thymosin beta-4 in cytoskeleton and cell migration assays.
What Equipment Is Needed Before Reconstitution?
Reconstitution needs the sealed blend vial, a preserved diluent, a graduated sterile syringe for measuring volume, alcohol swabs and a refrigerator that holds 2 to 8 °C.
- The blend vial, sealed, with its lot number entered in the laboratory notebook
- Bacteriostatic water preserved with 9 mg per mL benzyl alcohol, so the stock can be sampled on more than one day
- A 1 mL U-100 syringe for measuring 1 to 2 mL, or two measured fills for larger volumes
- Alcohol swabs for both stoppers
- A fine marker and a refrigerator verified at 2 to 8 °C
A laboratory starting without consumables can order the BPC-157 TB-500 starter kit, which packs the 5+5mg vial together with a 3 mL preserved diluent, twenty swabs and ten 0.5 mL syringes.
Reconstitution Procedure, Step by Step
1. Equilibrate the Vial
A cold vial collects condensation and the cake wets unevenly. Leave it capped on the bench for about 15 minutes.
2. Prepare the Stoppers and Measure the Diluent
Remove the flip caps, wipe each rubber stopper once with a swab and allow it to air dry. Draw the chosen volume slowly so no air bubble distorts the reading.
3. Add the Diluent Along the Glass
Tilt the vial and let the water run down the side wall rather than onto the powder. Blend vials are sealed under a mild vacuum, so the plunger may advance by itself.
4. Swirl, Do Not Shake
Roll the vial gently until no flakes remain. Shaking entrains air and can foam the solution. The 10+10mg cake carries twice the solids and may need several extra minutes; a short rest upright followed by another swirl normally clears it.
5. Label and Refrigerate
Mark the vial with the date, the millilitres added and the strength of each peptide, then return it to cold storage immediately.
How Much Bacteriostatic Water Goes Into Each Blend Vial?
Most laboratories add 2 mL to the 5+5mg vial and between 2 and 4 mL to the 10+10mg vial, according to the working concentration the protocol specifies. Concentration is milligrams divided by millilitres. On a U-100 syringe, 100 units equal 1 mL, so one unit is 0.01 mL. Multiply the strength in mg per mL by 0.01 to find the milligrams in one unit, then by 1,000 to give micrograms. Because each blend contains equal weights, every per-peptide figure is exactly half the combined figure.
| Vial | Water added | Combined strength | Each peptide | Each peptide per unit (0.01 mL) |
|---|---|---|---|---|
| 5+5mg | 1 mL | 10 mg per mL | 5 mg per mL | 50 mcg |
| 5+5mg | 2 mL | 5 mg per mL | 2.5 mg per mL | 25 mcg |
| 5+5mg | 2.5 mL | 4 mg per mL | 2 mg per mL | 20 mcg |
| 5+5mg | 3 mL | 3.33 mg per mL | 1.67 mg per mL | 16.7 mcg |
| 10+10mg | 2 mL | 10 mg per mL | 5 mg per mL | 50 mcg |
| 10+10mg | 3 mL | 6.67 mg per mL | 3.33 mg per mL | 33.3 mcg |
| 10+10mg | 4 mL | 5 mg per mL | 2.5 mg per mL | 25 mcg |
| 10+10mg | 5 mL | 4 mg per mL | 2 mg per mL | 20 mcg |
Before you reconstitute a BPC-157 + TB-500 blend at a larger volume, confirm the vial has headspace for it. If 4 or 5 mL will not fit, reconstitute the larger vial at 2 mL and transfer a measured portion into a second sterile vial for dilution, recording the new strength. The online peptide calculator handles any other volume.
Does Equal Weight Mean Equal Molecules?
Equal weight does not mean equal molecules, because the two peptides differ considerably in size. Dividing milligrams by molecular weight gives the amount in micromoles.
| Compound in 5mg | Approximate molecular weight | Amount in micromoles |
|---|---|---|
| BPC-157 | 1,419 Da | about 3.5 |
| Thymosin beta-4, full chain | 4,963 Da | about 1.0 |
| Ac-LKKTETQ fragment | about 890 Da | about 5.6 |
Where an assay is interpreted per mole rather than per milligram, the form stated on the certificate moves the ratio from roughly 3.5 to 1 in favour of BPC-157 to roughly 1 to 1.6 the other way. Record the form in the method section.
Can BPC-157 and TB-500 Be Mixed From Separate Vials?
Yes, separate vials of BPC-157 and TB-500 can each be reconstituted and then combined by measured volume, which permits any ratio and single-compound control arms. Protocols that need a ratio other than one to one start from individual vials of BPC-157 and TB-500. Joining equal volumes halves the concentration of each: 0.5 mL each of BPC-157 stock and TB-500 stock, both at 5 mg per mL, gives 1 mL containing 2.5 mg per mL of each.
| Consideration | Blend vial | Separate vials |
|---|---|---|
| Ratio | Fixed at one to one by weight | Any ratio the protocol sets |
| Lots to document | One | Two |
| Single-peptide controls | Not available | Available |
| Handling steps | One reconstitution | Two plus a combining step |
Our recommendation: use the blend when the protocol calls for the pair at one to one, reconstitute the 5+5mg vial with 2 mL so each unit holds a round 25 mcg of each peptide, and keep separate vials for single-agent arms.
How Long Does a Reconstituted Blend Last?
A blend reconstituted with bacteriostatic water and held in the dark between 2 and 8 °C is normally used within about 28 days, the standard in-use convention for preserved multi-use vials. The dry cake is most stable at -20 °C away from light and tolerates a few days at room temperature in transit. Water shortens that timeline considerably.
| State | Storage | Working window |
|---|---|---|
| Unopened freeze-dried vial | Freezer, -20 °C | Until the retest date on the certificate |
| Unopened freeze-dried vial | Refrigerator, 2 to 8 °C | Several weeks |
| Reconstituted with bacteriostatic water | Refrigerator, 2 to 8 °C, dark | About 28 days |
| Reconstituted with sterile water | Refrigerator, 2 to 8 °C | Within the same session |
Do not freeze the working solution. For studies longer than a month, divide a fresh stock into single-use aliquots on day one and freeze those, so no portion is thawed twice. The wider tissue repair research range follows the same storage rules.
Common Errors With Blend Vials
- Reading the combined strength as the strength of each peptide
- Assuming the TB-500 component is the full 43 residue chain without checking the certificate
- Adding 5 mL to a vial that cannot hold it
- Leaving the vial at room temperature between withdrawals
- Using sterile water for a stock intended to last several weeks
For the unit markings and barrel sizes that make those readings precise, see which syringe size suits peptide diluent measurement.
Questions about reconstitute a BPC-157 + TB-500 blend
Sources
- Complete amino acid sequence of bovine thymosin beta 4: a thymic hormone that induces terminal deoxynucleotidyl transferase activity in thymocyte populations. Proceedings of the National Academy of Sciences. 1981.
- Pharmacokinetics, distribution, metabolism, and excretion of body-protective compound 157, a potential drug for treating various wounds, in rats and dogs. Frontiers in Pharmacology. 2022.
Written by the Macropus Peptides technical team for in-vitro laboratory research reference. Not medical advice; products are not for human or veterinary use and are sold to buyers aged 18 and over.