Guide · 9 min read · Updated 30 September 2026
To reconstitute CJC-1295 and Ipamorelin, add a measured volume of bacteriostatic water slowly down the inner wall of the 10mg blend vial and swirl gently until the lyophilised cake dissolves. Two millilitres of diluent yields a combined stock of 5 mg per mL, equal to 50 mcg of blended peptide in every U-100 graduation. The solution is then labelled with its date and strength and held in a dark refrigerator between 2 and 8 °C.
Macropus Peptides supplies both compounds freeze-dried together as the CJC-1295 and Ipamorelin 10mg no DAC blend, with every lot HPLC verified at 98% or higher and its identity established by mass spectrometry. The method, arithmetic and storage conditions that follow apply to any freeze-dried blend of the two peptides.
A CJC-1295 and Ipamorelin blend vial holds two distinct peptides co-lyophilised in a single freeze-dried cake. CJC-1295 without DAC, also called modified GRF 1-29, is a 29 residue synthetic GHRH analogue carrying four substitutions (D-Ala2, Gln8, Ala15 and Leu27) that slow cleavage by dipeptidyl peptidase-4. Its molecular weight is approximately 3,368 daltons. Ipamorelin is a pentapeptide of about 712 daltons that acts at the ghrelin receptor, and it its selectivity as a growth hormone secretagogue was first described in a 1998 paper (European Journal of Endocrinology).
Because the two compounds act at separate pituitary receptors, the GHRH receptor and GHS-R1a, they are frequently examined together in secretagogue research. Each vial label states the fill weight of each peptide, the lot number and the storage temperature. The fill split printed on the label determines how many micrograms of each compound are present in any volume drawn, so it should be read from the label rather than assumed.
Reconstitution requires the sealed vial, a preserved diluent, a graduated syringe, isopropyl swabs and a fridge verified at 2 to 8 °C.
Laboratories equipping a bench for the first time often order the CJC-1295 Ipamorelin starter kit, which pairs the blend vial with a 3 mL bottle of preserved diluent, ten 0.5 mL syringes and twenty swabs.
The reconstitution method has five stages: bring the vial to ambient temperature, swab both stoppers, add the diluent slowly, swirl without shaking, then label and refrigerate the solution.
A vial taken directly from the freezer collects condensation and dissolves unevenly. Allow 15 to 20 minutes on the bench with the cap in place.
Remove the plastic caps, swab each rubber stopper and allow the alcohol to evaporate. Draw the selected volume of bacteriostatic water. Two millilitres is the usual choice because it produces round figures on a U-100 scale.
Aim the needle so the diluent runs down the inside of the glass instead of striking the cake, since freshly wetted lyophilised peptide foams under a direct jet. Vials are sealed under slight vacuum, so the plunger may draw inward; allow it to travel at its own rate.
Roll the vial between the fingers or swirl it in small circles. Most blends clear within two minutes. A solution that remains hazy can stand upright at room temperature for ten minutes before a second swirl. Persistent particles are grounds to quarantine the vial and contact us with the lot number.
Record the date, the volume added and the resulting concentration in mg per mL on the vial, then transfer it to 2 to 8 °C.
Concentration equals fill weight in milligrams divided by diluent volume in millilitres. A U-100 barrel carries 100 graduations per millilitre, making each graduation 0.01 mL, and the peptide in one unit is the concentration multiplied by 0.01 mL.
| Diluent added | Peptide per 0.01 mL (1 unit) | Combined concentration | Peptide per 0.1 mL (10 units) |
|---|---|---|---|
| 1 mL | 100 mcg | 10 mg per mL | 1,000 mcg |
| 2 mL | 50 mcg | 5 mg per mL | 500 mcg |
| 2.5 mL | 40 mcg | 4 mg per mL | 400 mcg |
| 3 mL | 33.3 mcg | 3.33 mg per mL | 333 mcg |
| 4 mL | 25 mcg | 2.5 mg per mL | 250 mcg |
| 5 mL | 20 mcg | 2 mg per mL | 200 mcg |
The chart gives combined peptide for anyone who needs to reconstitute CJC-1295 and Ipamorelin from the 10mg blend. Each compound's share is found by multiplying by its fraction of the labelled fill. For a lot labelled 5mg of each peptide, half of every figure belongs to each compound, so 1 unit at the 2 mL concentration contains 25 mcg of CJC-1295 and 25 mcg of Ipamorelin. Our online peptide calculator repeats these sums for any fill weight and diluent volume a protocol specifies.
Most laboratory protocols reconstitute the 10mg blend with 2 to 3 mL of preserved diluent, balancing measurement precision against the time the stock spends in use. A smaller volume produces a stronger stock and smaller draws, which magnifies any reading error on the barrel. A larger volume lowers the peptide per unit and improves precision, but the stock is drawn more times and remains in refrigerated storage for longer, closer to the 28 day in-use limit many laboratories apply.
Yes, the two peptides tolerate each other in solution and can share one vial, either as a co-lyophilised blend or by pooling two separately reconstituted stocks. A blended vial fixes the ratio at manufacture under a single lot number. The alternative is to reconstitute a CJC-1295 no DAC vial and an Ipamorelin 10mg vial independently, then combine measured volumes.
Separate vials permit any ratio and support single-compound control arms. They also introduce a dilution effect that is easily overlooked: pooling half a millilitre from each of two stocks prepared at 5 mg per mL leaves each peptide at 2.5 mg per mL, because the total volume has doubled. The final concentration of each compound, not the stock concentration, is the figure to record.
| Consideration | Blended vial | Two separate vials |
|---|---|---|
| Ratio | Fixed, printed on the label | Set by the researcher |
| Lot numbers to track | One | Two |
| Single-compound control arms | Not possible | Possible |
| Reconstitutions required | One | Two |
| Risk of arithmetic error | Low | Higher |
Our recommendation for most laboratories is the blended vial for combined-signal work, with separate vials reserved for protocols that require each compound in isolation.
After mixing, the CJC-1295 and Ipamorelin solution belongs in refrigerated storage between 2 and 8 °C, standing upright in its carton, and should be used within the in-use period the protocol sets. Many laboratories apply a 28 day limit to a bacteriostatic stock, a figure adopted from the convention for opened multi-use vials of preserved water. Unopened freeze-dried powder keeps longest frozen at -20 °C with light excluded, and tolerates several days at ambient temperature in transit.
| Form | Temperature | Typical handling window |
|---|---|---|
| Sealed lyophilised vial | -20 °C | Long term, to the date on the certificate |
| Sealed lyophilised vial | 2 to 8 °C | Weeks |
| Sealed vial in transit | Ambient | Days |
| Reconstituted in bacteriostatic water | 2 to 8 °C | Up to about 28 days by laboratory convention |
| Reconstituted in sterile water | 2 to 8 °C | Single session, then discard |
Freezing the reconstituted working solution is not recommended, because ice formation and repeated thawing can drive peptide chains into aggregates. Aggregated material no longer behaves as the original compound, and no simple bench test detects the change. Where a longer study requires frozen stock, a fresh solution can be divided into single-use aliquots on the day of reconstitution and frozen once, with no aliquot refrozen after thawing.
Every peptide in our growth hormone research range follows the same handling principles, so the routine transfers directly to other secretagogues and releasing hormone analogues. For a storage question in the metabolic range, see how long retatrutide lasts once mixed.
Written by the Macropus Peptides technical team for in-vitro laboratory research reference. Not medical advice; products are not for human or veterinary use and are sold to buyers aged 18 and over.
